Recombinant Human CCR4-NOT transcription complex subunit 6-like (CNOT6L), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 69.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human CCR4-NOT transcription complex subunit 6-like (CNOT6L)

Recombinant Human CCR4-NOT transcription complex subunit 6-like (CNOT6L) — Has 3′-5′ poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Purity >85%.

SKU: BCRECP-000395 Category:
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Product Specifications

Product SkuBCRECP-000395
Product DescriptionRecombinant Human CCR4-NOT transcription complex subunit 6-like (CNOT6L) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-555aa. [Accession | Q96LI5].
Uniprot No.Q96LI5
Gene NamesCNOT6L
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-555aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight69.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Highly expressed in placenta, skeletal muscle, pancreas, testis and leukocytes. Weakly expressed in heart, spleen and thymus

Product Specifications

Recombinant Human CCR4-NOT transcription complex subunit 6-like (CNOT6L) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-555aa, with N-terminal 10xHis-tagged tag, molecular weight 69.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in the deadenylation-dependent degradation of mRNAs through the 3'-UTR AU-rich element-mediated mechanism. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Highly expressed in placenta, skeletal muscle, pancreas, testis and leukocytes. Weakly expressed in heart, spleen and thymus

Subunit

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (PubMed:17452450, PubMed:18377426, PubMed:19558367). Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (PubMed:17452450). Interacts with TOB1 (PubMed:18377426). Interacts with NANOS2 (By similarity). Interacts with ZFP36 (By similarity). Interacts with ZFP36L2 (PubMed:34611029). Interacts with RBM46 (By similarity)

Gene: CNOT6L  |  Organism: Homo sapiens  |  Synonyms: Carbon catabolite repressor protein 4 homolog B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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