Recombinant Human Ciliary neurotrophic factor (CNTF), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 29kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Ciliary neurotrophic factor (CNTF)

Recombinant Human Ciliary neurotrophic factor (CNTF) — Functions as a cytokine through the ciliary neurotrophic factor receptor (CNTFR) complex. Purity >95%.

SKU: BCRECP-000397 Category:
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Product Specifications

Product SkuBCRECP-000397
Product DescriptionRecombinant Human Ciliary neurotrophic factor (CNTF) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-200aa. [Accession | P26441].
Uniprot No.P26441
Gene NamesCNTF
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-200aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight29kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Functions as a cytokine through the ciliary neurotrophic factor receptor (CNTFR) complex. The CNTFR complex indeed consists of the membrane-anchored non-signaling receptor CNTFR and the two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell. Functionally, promotes neuronal survival, supports differentiation of various neuronal and non-neuronal cells, and importantly regulates oligodendrocyte progenitor proliferation.

Biological Context

Subcellular Location: Cytoplasm; Secreted

Product Specifications

Recombinant Human Ciliary neurotrophic factor (CNTF) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-200aa, with N-terminal 10xHis-tagged tag, molecular weight 29kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Functions as a cytokine through the ciliary neurotrophic factor receptor (CNTFR) complex (PubMed:10966616, PubMed:11285233, PubMed:11294841, PubMed:31932351, PubMed:36930708). The CNTFR complex indeed consists of the membrane-anchored non-signaling receptor CNTFR and the two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell (PubMed:36930708). Functionally, promotes neuronal survival, supports differentiation of various neuronal and non-neuronal cells, and importantly regulates oligodendrocyte progenitor proliferation (PubMed:1861138, PubMed:1883844, PubMed:31932351). Mechanistically, ligand binding to the CNTFR induces dimerization of the IL6ST/gp130 and LIFR, which activates JAK tyrosine kinases (JAK1 or JAK2 and to lesser extent TYK2) bound to their intracellular domains (PubMed:11294841). These kinases subsequently phosphorylate IL6ST/gp130 and LIFR (PubMed:11294841). The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of signal transducer and activators of transcription (STAT3 and to lesser extent STAT1) (PubMed:11285233, PubMed:11294841). Can also signal via trans-signaling using soluble forms of CNTFR (PubMed:31932351, PubMed:7681218). Seems to prevent the degeneration of motor axons after axotomy (By similarity)

Subcellular Location

Cytoplasm; Secreted

Subunit

Homodimer

Gene: CNTF  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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