Function
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Product Specifications
Recombinant Human Coagulation factor IX (F9) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 29-461aa, with C-terminal 10xHis-tagged tag, molecular weight 50.1 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 2.428-2.771 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 4.451-5.233 ng/mL.;GPC3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis GPC3 with an affinity constant of 0.392 nM as detected by MetaSPR Assay (WeSPRTM 200).;GPC3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis GPC3 with an affinity constant of 0.228 nM as detected by MetaSPR Assay (WeSPRTM 200).
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
