Recombinant Human coronavirus 229E Envelope small membrane protein (E) Protein, Human coronavirus 229E (HCoV-229E), in vitro E.coli expression system, N-terminal 10xHis-tagged, 10.6kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human coronavirus 229E Envelope small membrane protein (E) Protein

Recombinant Human coronavirus 229E Envelope small membrane protein (E) Protein — Plays a central role in virus morphogenesis and assembly. Purity >95%.

SKU: BCRECP-00057TM Categories: , , ,
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Product Specifications

Uniprot No.P19741
Gene NamesE
PurityGreater than 95% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-77aa
SpeciesHuman coronavirus 229E (HCoV-229E)
Tag InfoN-terminal 10xHis-tagged
Molecular weight10.6kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis.

Biological Context

Subcellular Location: Host Golgi apparatus membrane (Single-pass type III membrane protein)

Product Specifications

Recombinant Human coronavirus 229E Envelope small membrane protein (E) Protein is a recombinant protein from Human coronavirus 229E (HCoV-229E), expressed in in vitro E.coli expression system, covering amino acids 1-77aa, with N-terminal 10xHis-tagged tag, molecular weight 10.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis

Subcellular Location

Host Golgi apparatus membrane (Single-pass type III membrane protein)

Subunit

Homopentamer. Interacts with membrane protein M in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex. Interacts with Nucleoprotein

Gene: E  |  Organism: Human coronavirus 229E

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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