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Recombinant Human coronavirus HKU1 Protein I (N)

Recombinant Human coronavirus HKU1 Protein I (N) — recombinant protein from Human coronavirus HKU1 (isolate N5) (HCoV-HKU1), expressed in E.coli. Purity >85%.

Product Specifications

Product SkuBCRECP-003490
Product DescriptionRecombinant Human coronavirus HKU1 Protein I (N) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-205aa. [Accession | Q0ZME2].
Uniprot No.Q0ZME2
Gene NamesN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-205aa
SpeciesHuman coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Structural protein that is not essential for the viral replication either in tissue culture or in its natural host.

Product Specifications

Recombinant Human coronavirus HKU1 Protein I (N) — recombinant protein from Human coronavirus HKU1 (isolate N5) (HCoV-HKU1), expressed in E.coli. Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Structural protein that is not essential for the viral replication either in tissue culture or in its natural host

Subcellular Location

Virion

Gene: N  |  Organism: Human coronavirus HKU1 (isolate N5)  |  Synonyms: Accessory protein N2; N internal ORF protein; Orf8 protein; Protein in nucleocapsid ORF

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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