Recombinant Human Corticotropin-releasing factor receptor 2 (CRHR2) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 49.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Corticotropin-releasing factor receptor 2 (CRHR2) Protein

Recombinant Human Corticotropin-releasing factor receptor 2 (CRHR2) Protein — G protein-coupled receptor for CRH, UCN, UCN2 and UCN3 stress hormones, which plays a central role in whole body adaptation to stress. Purity >85%.

SKU: BCRECP-00166TM Category:
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Product Specifications

Uniprot No.Q13324
Gene NamesCRHR2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-411aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight49.1kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor for CRH, UCN, UCN2 and UCN3 stress hormones, which plays a central role in whole body adaptation to stress. Has highest affinity for UCN, and much lower affinity for CRH, UNC2 and UCN3. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and downstream effectors, such as adenylate cyclase.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Human Corticotropin-releasing factor receptor 2 (CRHR2) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-411aa, with N-terminal 10xHis-tagged tag, molecular weight 49.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for CRH, UCN, UCN2 and UCN3 stress hormones, which plays a central role in whole body adaptation to stress (PubMed:11329063, PubMed:11416224, PubMed:20966082, PubMed:22689579, PubMed:32004470, PubMed:36335102). Has highest affinity for UCN, and much lower affinity for CRH, UNC2 and UCN3 (PubMed:20966082, PubMed:22689579, PubMed:32004470, PubMed:36335102). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and downstream effectors, such as adenylate cyclase (PubMed:32004470, PubMed:36335102). CRHR2 is coupled to G(s) G protein (GNAS), mediating activation of adenylate cyclase activity and production of cAMP (PubMed:32004470, PubMed:36335102). CRHR2-dependent signaling is a primary mediator of the neuroendocrine, autonomic (fight-or-flight) and behavioral responses to stress, acting as a key regulator of adaptation by activating the hypothalamus-pituitary-adrenal axis, leading to corticotropin hormone (ACTH) production (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Monomer

Gene: CRHR2  |  Organism: Homo sapiens  |  Synonyms: Corticotropin-releasing factor receptor 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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