Recombinant Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 39.1kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)

Recombinant Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) — Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids. Purity >95%.

SKU: BCRECP-000553 Categories: ,
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Product Specifications

Product SkuBCRECP-000553
Product DescriptionRecombinant Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 34-328aa. [Accession | Q13011].
Uniprot No.Q13011
Gene NamesECH1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region34-328aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight39.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids. Together with the 2,4-dienoyl-CoA reductase (DECR) and the Enoyl-CoA delta isomerase 1 (ECI1) they allow reentrance of the enoyl-CoA into the beta-oxidation cycle. Able to isomerize 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position.

Biological Context

Subcellular Location: Mitochondrion; Peroxisome
Pathway: Lipid metabolism; fatty acid beta-oxidation

Product Specifications

Recombinant Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 34-328aa, with C-terminal 6xHis-tagged tag, molecular weight 39.1kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:26474213). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Enoyl-CoA delta isomerase 1 (ECI1) they allow reentrance of the enoyl-CoA into the beta-oxidation cycle (PubMed:26474213). Able to isomerize 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position (By similarity) (PubMed:26474213). Based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (Probable)

Subcellular Location

Mitochondrion; Peroxisome

Subunit

Homohexamer

Pathway

Lipid metabolism; fatty acid beta-oxidation

Gene: ECH1  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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