Recombinant Human Density-regulated protein (DENR)

Function

Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent

Subcellular Location

Cytoplasm

Tissue Specificity

Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney

Subunit

Interacts with MCTS1 (via PUA domain); the complex regulates translation reinitiation

• Deyo J.E. et al. Drp, a novel protein expressed at high cell density but not during growth arrest. — DNA Cell Biol. (1998)
• Nishimoto S. et al. Molecular cloning and characterization of human smooth muscle cell associated protein-3 (SMAP-3). (MAY-)
• Scherer S.E. et al. The finished DNA sequence of human chromosome 12. — Nature (2006)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Oh J.J. et al. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. — Nucleic Acids Res. (1999)
• Reinert L.S. et al. MCT-1 protein interacts with the cap complex and modulates messenger RNA translational profiles. — Cancer Res. (2006)
• Olsen J.V. et al. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. — Cell (2006)
• Mazan-Mamczarz K. et al. Post-transcriptional control of the MCT-1-associated protein DENR/DRP by RNA-binding protein AUF1. — Cancer Genomics Proteomics (2007)