Recombinant Human Diacylglycerol kinase alpha (DGKA), Homo sapiens (Human), E.coli, N-terminal 6XHis-tagged, 86.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Diacylglycerol kinase alpha (DGKA)

Recombinant Human Diacylglycerol kinase alpha (DGKA) — Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Purity >90%.

SKU: BCRECP-000502 Categories: , ,
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Product Specifications

Product SkuBCRECP-000502
Product DescriptionRecombinant Human Diacylglycerol kinase alpha (DGKA) Protein is expressed from E.coli with N-terminal 6XHis-tagged. It contains 1-735aa. [Accession | P23743].
Uniprot No.P23743
Gene NamesDGKA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-735aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6XHis-tagged
Molecular weight86.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable).

Biological Context

Subcellular Location: Cytoplasm, cytosol
Tissue Specificity: Expressed in lymphocytes
Pathway: Lipid metabolism; glycerolipid metabolism

Product Specifications

Recombinant Human Diacylglycerol kinase alpha (DGKA) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-735aa, with N-terminal 6XHis-tagged tag, molecular weight 86.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129)

Subcellular Location

Cytoplasm, cytosol

Tissue Specificity

Expressed in lymphocytes

Subunit

Monomer

Pathway

Lipid metabolism; glycerolipid metabolism

Gene: DGKA  |  Organism: Homo sapiens  |  Synonyms: 80 kDa diacylglycerol kinase; Diglyceride kinase alpha
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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