Recombinant Human Dihydroorotate dehydrogenase (quinone),mitochondrial (DHODH), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 45.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Dihydroorotate dehydrogenase (quinone),mitochondrial (DHODH), partial

Recombinant Human Dihydroorotate dehydrogenase (quinone),mitochondrial (DHODH), partial — Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Purity >85%.

SKU: BCRECP-000505 Categories: ,
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Product Specifications

Product SkuBCRECP-000505
Product DescriptionRecombinant Human Dihydroorotate dehydrogenase (quinone) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 31-395aa. [Accession | Q02127].
Uniprot No.Q02127
Gene NamesDHODH
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region31-395aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight45.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.

Biological Context

Subcellular Location: Mitochondrion inner membrane (Single-pass membrane protein)
Disease Association: Postaxial acrofacial dysostosis (POADS) : An autosomal recessive syndrome characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1

Product Specifications

Recombinant Human Dihydroorotate dehydrogenase (quinone),mitochondrial (DHODH), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 31-395aa, with N-terminal 10xHis-tagged tag, molecular weight 45.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway

Subcellular Location

Mitochondrion inner membrane (Single-pass membrane protein)

Disease Association

Postaxial acrofacial dysostosis (POADS) : An autosomal recessive syndrome characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Monomer

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1

Gene: DHODH  |  Organism: Homo sapiens  |  Synonyms: Dihydroorotate oxidase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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