Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 39.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A)

Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A) — DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Purity >90%.

SKU: BCRECP-000107 Categories: ,
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Product Specifications

Product SkuBCRECP-000107
Product DescriptionRecombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-199aa. [Accession | P31941].
Uniprot No.P31941
Gene NamesAPOBEC3A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-199aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight39.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA.

Biological Context

Subcellular Location: Nucleus; Cytoplasm
Tissue Specificity: (Microbial infection) Expression in the urothelium is increased upon infection by BK polyomavirus (BKPyV)
Disease Association: [In the urothelium, antiviral activity of APOBEC3A and APOBEC3B against BK polyomavirus (BKPyV) drives a high burden of mutations in cells proximal to infected neighbors (transmutagenesis), and may be involved in urothelial carcinogenesis]

Product Specifications

Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-199aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 39.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation

Subcellular Location

Nucleus; Cytoplasm

Disease Association

[In the urothelium, antiviral activity of APOBEC3A and APOBEC3B against BK polyomavirus (BKPyV) drives a high burden of mutations in cells proximal to infected neighbors (transmutagenesis), and may be involved in urothelial carcinogenesis]

Tissue Specificity

(Microbial infection) Expression in the urothelium is increased upon infection by BK polyomavirus (BKPyV)

Subunit

Interacts with AGO2. Interacts with TRIB3 (via N-terminus)

Gene: APOBEC3A  |  Organism: Homo sapiens  |  Synonyms: Phorbolin-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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