Recombinant Human Dynactin subunit 3 (DCTN3), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 35.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Dynactin subunit 3 (DCTN3)

Recombinant Human Dynactin subunit 3 (DCTN3) — Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Purity >90%.

SKU: BCRECP-000483 Category:
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Product Specifications

Product SkuBCRECP-000483
Product DescriptionRecombinant Human Dynactin subunit 3 (DCTN3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-176aa. [Accession | O75935].
Uniprot No.O75935
Gene NamesDCTN3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-176aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight35.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Cycle

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Together with dynein may be involved in spindle assembly and cytokinesis.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle; Cleavage furrow; Midbody
Tissue Specificity: Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain

Product Specifications

Recombinant Human Dynactin subunit 3 (DCTN3) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-176aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 35.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Together with dynein may be involved in spindle assembly and cytokinesis (PubMed:9722614)

Subcellular Location

Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle; Cleavage furrow; Midbody

Tissue Specificity

Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain

Subunit

Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin

Gene: DCTN3  |  Organism: Homo sapiens  |  Synonyms: Dynactin complex subunit 22 kDa subunit
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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