Recombinant Human Dynein light chain 1, Cytoplasmic domain (DYNLL1), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 37.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Dynein light chain 1, Cytoplasmic domain (DYNLL1)

Recombinant Human Dynein light chain 1, Cytoplasmic domain (DYNLL1) — Component of dynein, a family of motor proteins essential for movement along microtubules. Purity >90%.

SKU: BCRECP-000552 Category:
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Product Specifications

Product SkuBCRECP-000552
Product DescriptionRecombinant Human Dynein light chain 1, Cytoplasmic domain (DYNLL1) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-89aa. [Accession | P63167].
Uniprot No.P63167
Gene NamesDYNLL1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-89aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight37.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasApoptosis

Function

Component of dynein, a family of motor proteins essential for movement along microtubules. Required for structural and functional integrity of cilia. Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.

Biological Context

Subcellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome; Cytoplasm, cytoskeleton; Nucleus; Mitochondrion
Tissue Specificity: Ubiquitous. Expressed in testis

Product Specifications

Recombinant Human Dynein light chain 1, Cytoplasmic domain (DYNLL1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-89aa, with N-terminal GST-tagged tag, molecular weight 37.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of dynein, a family of motor proteins essential for movement along microtubules (By similarity). Required for structural and functional integrity of cilia (By similarity). Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function (By similarity). Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules (By similarity). May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity). In addition to its role in cytoskeleton and transport, acts as a protein-protein adapter, which inhibits and/or sequesters target proteins (PubMed:10198631, PubMed:15193260, PubMed:15891768, PubMed:16684779, PubMed:30464262, PubMed:37696958). Involved in the response to DNA damage by acting as a key regulator of DNA end resection: when phosphorylated at Ser-88, recruited to DNA double-strand breaks (DSBs) by TP53BP1 and acts by disrupting MRE11 dimerization, thereby inhibiting DNA end resection (PubMed:30464262, PubMed:37696958). In a subset of DSBs, DYNLL1 remains unphosphorylated and promotes the recruitment of the Shieldin complex (PubMed:37696958). Binds and inhibits the catalytic activity of neuronal nitric oxide synthase/NOS1 (By similarity). Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1 (PubMed:15891768, PubMed:16684779). Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules (PubMed:10198631, PubMed:15193260). Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity (PubMed:10198631, PubMed:15193260)

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome; Cytoplasm, cytoskeleton; Nucleus; Mitochondrion

Tissue Specificity

Ubiquitous (PubMed:8628263). Expressed in testis (PubMed:22965910)

Subunit

(Microbial infection) Interacts with ebolavirus protein VP35; this interaction stabilizes VP35 N-terminal oligomerization domain and enhances viral RNA synthesis

Gene: DYNLL1  |  Organism: Homo sapiens  |  Synonyms: 8 kDa dynein light chain; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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