Recombinant Human E3 ubiquitin-protein ligase RNF5 (RNF5) Protein

Function

Membrane-bound E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins (PubMed:11329381, PubMed:12861019, PubMed:16176924, PubMed:19269966, PubMed:19285439). May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4 (PubMed:11329381). Mediates ubiquitination of PXN/paxillin,thereby regulating cell motility and localization of PXN/paxillin (PubMed:12861019). Catalyzes ubiquitination of Salmonella type III secreted protein sopA (PubMed:16176924). Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N (PubMed:19269966). Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses (PubMed:19285439). Catalyzes ubiquitination and subsequent degradation of ATG4B, thereby inhibiting autophagy (PubMed:23093945)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Mitochondrion membrane (Multi-pass membrane protein); Endoplasmic reticulum membrane (Multi-pass membrane protein)

Tissue Specificity

Widely expressed

Subunit

Interacts with PXN (PubMed:12861019). Interacts with Salmonella typhimurium sopA (PubMed:12861019). Interacts with JKAMP (PubMed:19269966). Interacts with STING1; the interaction of endogenous proteins is dependent on viral infection (PubMed:19285439)

Pathway

Protein modification; protein ubiquitination

• Kyushiki H. et al. Cloning, expression and mapping of a novel RING-finger gene (RNF5), a human homologue of a putative zinc-finger gene from Caenorhabditis elegans. — Cytogenet. Cell Genet. (1997)
• Matsuda N. et al. Rma1, a novel type of RING finger protein conserved from Arabidopsis to human, is a membrane-bound ubiquitin ligase. — J. Cell Sci. (2001)
• Khanna A. et al. Characterisation of a novel gene, G16, in the class III region of the human major histocompatibility complex. (JUL-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Kalnine N. et al. Cloning of human full-length CDSs in BD Creator(TM) system donor vector. (MAY-)
• Xie T. et al. Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse. — Genome Res. (2003)
• Mungall A.J. et al. The DNA sequence and analysis of human chromosome 6. — Nature (2003)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)