Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 20.4 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3), partial (Active)

Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3), partial (Active) — E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and… Purity >95%.

SKU: BCACP-000162 Categories: ,
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Product SKUBCACP-000162
Product DescriptionRecombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 56-219aa. [Accession | Q9ULT6].
Uniprot No.Q9ULT6
Gene NamesZNRF3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region56-219aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight20.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 10 μg/mL can bind Anti-CCR4 recombinant antibody, the EC50 is 362.3-630.8 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(RING finger protein 203)(RING-type E3 ubiquitin transferase ZNRF3)(Zinc/RING finger protein 3)

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human E3 ubiquitin-protein ligase ZNRF3 (ZNRF3), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 56-219aa, with C-terminal 6xHis-tagged tag, molecular weight 20.4 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 10 μg/mL can bind Anti-CCR4 recombinant antibody, the EC50 is 362.3-630.8 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with LRP6, FZD4, FZD5, FZD6 and FZD8 (PubMed:22575959). Interacts with RSPO1; interaction promotes indirect interaction with LGR4 and membrane clearance of ZNRF3 (PubMed:22575959). Also interacts with RSPO2 (PubMed:29769720). Interacts with LMBR1L (PubMed:31073040)

Pathway

Protein modification; protein ubiquitination

Gene: ZNRF3  |  Organism: Homo sapiens  |  Synonyms: RING finger protein 203; RING-type E3 ubiquitin transferase ZNRF3; Zinc/RING finger protein 3
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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