Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)，partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 53.0 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)，partial (Active)

Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)，partial (Active) — Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (Probable). Purity >90%.

SKU: BCACP-000633 Categories: ,
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Product SKUBCACP-000633
Product DescriptionRecombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)， protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 38-478aa. [Accession | P49961].
Uniprot No.P49961
Gene NamesENTPD1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region38-478aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight53.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Mouse Fap at 2 μg/mL can bind Anti-FAP recombinant antibody . The EC50 is 0.7918-0.9101 ng/mL.;FAP Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Mouse Fap with an affinity constant of 1.51 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesEctonucleoside triphosphate diphosphohydrolase 1; EC 3.6.1.5; ATP diphosphohydrolase; ATP-DPH; ATPDase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; Nucleoside triphosphate diphosphohydrolase 1; NTPDase1; CD antigen CD39

Function

Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (Probable). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (Probable). This activity contributes to the regulation of extracellular levels of nucleotides (Probable).

Biological Context

Subcellular Location: Membrane (Multi-pass membrane protein); Membrane, caveola
Tissue Specificity: Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Highly expressed in placenta, lung, skeletal muscle, kidney
Disease Association: Spastic paraplegia 64, autosomal recessive (SPG64) : A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)，partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 38-478aa, with C-terminal 10xHis-tagged tag, molecular weight 53.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Fap at 2 μg/mL can bind Anti-FAP recombinant antibody . The EC50 is 0.7918-0.9101 ng/mL.;FAP Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Mouse Fap with an affinity constant of 1.51 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). This activity contributes to the regulation of extracellular levels of nucleotides (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed:8955160, PubMed:8996251)

Subcellular Location

Membrane (Multi-pass membrane protein); Membrane, caveola

Disease Association

Spastic paraplegia 64, autosomal recessive (SPG64) : A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed primarily on activated lymphoid cells (PubMed:7930580). Also expressed in endothelial tissues (PubMed:8955160). Highly expressed in placenta, lung, skeletal muscle, kidney (PubMed:8955160)

Subunit

Homodimer; disulfide-linked

Gene: ENTPD1  |  Organism: Homo sapiens  |  Synonyms: ATP diphosphohydrolase; Ecto-ATP diphosphohydrolase 1; Ecto-apyrase; Lymphoid cell activation antigen; Nucleoside triphosphate diphosphohydrolase 1
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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