Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)

Function

(Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection

Subcellular Location

Cytoplasm; Nucleus; Nucleus, nucleolus; Cell membrane

Subunit

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA (PubMed:12426392, PubMed:12426393). Interacts with PARP1 (PubMed:17177976). Interacts with KARS1 (PubMed:18029264). May interact with ERGIC2 (PubMed:17980171). Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation (PubMed:19158340). Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (PubMed:8650580). Interacts with PPP1R16B (PubMed:26497934). Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity (PubMed:18263879). Interacts with guanyl-nucleotide exchange factor EEF1B2 (By similarity). Interacts (via middle-region) with HTATIP2 (via N-terminus); the interaction is direct and competes with EEF1A1 binding to guanyl-nucleotide exchange factor EEF1B2, thereby inhibiting GDP for GTP exchange and reactivation of EEF1A1 (By similarity). Interacts with tRNA (By similarity). Interacts with CEP112 (PubMed:39349455)

• Brands J.H.G.M. et al. The primary structure of the alpha subunit of human elongation factor 1. Structural aspects of guanine-nucleotide-binding sites. — Eur. J. Biochem. (1986)
• Uetsuki T. et al. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha. — J. Biol. Chem. (1989)
• Madsen H.O. et al. Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family. — Nucleic Acids Res. (1990)
• Shimazu T. et al. Postnatal expression of a novel mRNA isoform from the human elongation factor-1a gene. (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Rao T.R. et al. Structure of the amino-terminal end of mammalian elongation factor Tu. — Nucleic Acids Res. (1986)
• Bienvenut W.V. et al. PubMed: CI-5MVDLDRD40150 (JAN-)
• Maruyama T. et al. Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells. — Clin. Exp. Immunol. (2007)