Recombinant Human Elongation factor Tu, mitochondrial (TUFM), Homo sapiens (Human), Yeast, N-terminal 6xHis-tagged, 47.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Elongation factor Tu, mitochondrial (TUFM)

Recombinant Human Elongation factor Tu, mitochondrial (TUFM) — GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Purity >90%.

SKU: BCRECP-001875 Category:
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Product Specifications

Product SkuBCRECP-001875
Product DescriptionRecombinant Human Elongation factor Tu, mitochondrial (TUFM) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 47-455aa. [Accession | P49411].
Uniprot No.P49411
Gene NamesTUFM
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region47-455aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight47.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation. Also plays a role in the regulation of autophagy and innate immunity.

Biological Context

Subcellular Location: Mitochondrion matrix
Disease Association: Combined oxidative phosphorylation deficiency 4 (COXPD4) : A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Protein biosynthesis; polypeptide chain elongation

Product Specifications

Recombinant Human Elongation factor Tu, mitochondrial (TUFM) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 47-455aa, with N-terminal 6xHis-tagged tag, molecular weight 47.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation (By similarity). Also plays a role in the regulation of autophagy and innate immunity (PubMed:22749352, PubMed:28407488). Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway (PubMed:28407488). In turn, inhibits RLR-mediated type I interferon while promoting autophagy (PubMed:22749352)

Subcellular Location

Mitochondrion matrix

Disease Association

Combined oxidative phosphorylation deficiency 4 (COXPD4) : A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

(Microbial infection) Interacts with Hantaan hantavirus glycoprotein N; this interaction contributes to the virus-induced degradation of mitochondria by autophagy, which leads to degradation of MAVS and inhibition of type I interferon (IFN) responses

Pathway

Protein biosynthesis; polypeptide chain elongation

Gene: TUFM  |  Organism: Homo sapiens  |  Synonyms: P43
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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