Recombinant Human Endophilin-B1 (SH3GLB1), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 47.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Endophilin-B1 (SH3GLB1)

Recombinant Human Endophilin-B1 (SH3GLB1) — May be required for normal outer mitochondrial membrane dynamics. Purity >90%.

SKU: BCRECP-001628 Category:
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Product Specifications

Product SkuBCRECP-001628
Product DescriptionRecombinant Human Endophilin-B1 (SH3GLB1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-365aa. [Accession | Q9Y371].
Uniprot No.Q9Y371
Gene NamesSH3GLB1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-365aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight47.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature.

Biological Context

Subcellular Location: Cytoplasm; Golgi apparatus membrane (Peripheral membrane protein); Mitochondrion outer membrane (Peripheral membrane protein); Cytoplasmic vesicle, autophagosome membrane; Midbody
Tissue Specificity: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes

Product Specifications

Recombinant Human Endophilin-B1 (SH3GLB1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-365aa, with C-terminal 6xHis-tagged tag, molecular weight 47.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity)

Subcellular Location

Cytoplasm; Golgi apparatus membrane (Peripheral membrane protein); Mitochondrion outer membrane (Peripheral membrane protein); Cytoplasmic vesicle, autophagosome membrane; Midbody

Tissue Specificity

Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes

Subunit

Homodimer, and heterodimer with SH3GLB2 (PubMed:11161816). Binds BAX; induction of apoptosis augments BAX binding (PubMed:11161816, PubMed:11259440). Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 (By similarity). Interacts with UVRAG; UVRAG bridges the interaction to BECN1 indicative for an association with the PI3K complex II (PI3KC3-C2) (PubMed:17891140, PubMed:20643123)

Gene: SH3GLB1  |  Organism: Homo sapiens  |  Synonyms: Bax-interacting factor 1; SH3 domain-containing GRB2-like protein B1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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