Recombinant Human Eukaryotic translation initiation factor 5B (EIF5B), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 30.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Eukaryotic translation initiation factor 5B (EIF5B), partial

Recombinant Human Eukaryotic translation initiation factor 5B (EIF5B), partial — Plays a role in translation initiation. Purity >90%.

SKU: BCRECP-000579 Category:
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Product Specifications

Product SkuBCRECP-000579
Product DescriptionRecombinant Human Eukaryotic translation initiation factor 5B (EIF5B) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 629-846aa. [Accession | O60841].
Uniprot No.O60841
Gene NamesEIF5B
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region629-846aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight30.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Human Eukaryotic translation initiation factor 5B (EIF5B), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 629-846aa, with N-terminal 10xHis-tagged tag, molecular weight 30.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Plays a role in translation initiation (PubMed:10659855, PubMed:35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed:10659855, PubMed:35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed:12569173, PubMed:35732735). Is released after formation of the 80S initiation complex (PubMed:35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes (PubMed:10659855, PubMed:35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed:10659855)

Subcellular Location

Cytoplasm

Subunit

Interacts through its C-terminal domain (CTD) with the CTD of eIF1A (EIF1AX) or with the CTD of EIF5 (mutually exclusive) through a common binding site (PubMed:30211544). Interacts with eIF1A (EIF1AX) from the location of the start codon by the 43S complex until the formation of the 80S complex (PubMed:12569173, PubMed:35732735). Interacts with ANXA5 in a calcium and phospholipid-dependent manner (By similarity)

Gene: EIF5B  |  Organism: Homo sapiens  |  Synonyms: Translation initiation factor IF-2
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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