Recombinant Human F-box only protein 44 (FBXO44), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 37.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human F-box only protein 44 (FBXO44)

Recombinant Human F-box only protein 44 (FBXO44) — Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex. Purity >85%.

SKU: BCRECP-000639 Category:
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Product Specifications

Product SkuBCRECP-000639
Product DescriptionRecombinant Human F-box only protein 44 (FBXO44) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-255aa. [Accession | Q9H4M3].
Uniprot No.Q9H4M3
Gene NamesFBXO44
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-255aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight37.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areascell Biology

Function

Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex. The complex SCF(FBXO44) complex ubiquitinates and mediates BRCA1 degradation. In association with cullin 4B/CUL4B and DDB1, mediates the ubiquitination of RGS2 leading to its degradation.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver

Product Specifications

Recombinant Human F-box only protein 44 (FBXO44) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-255aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 37.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex (PubMed:18203720, PubMed:25970626). The complex SCF(FBXO44) complex ubiquitinates and mediates BRCA1 degradation (PubMed:23086937). In association with cullin 4B/CUL4B and DDB1, mediates the ubiquitination of RGS2 leading to its degradation (PubMed:25970626). Additionally, regulates the pregnane X receptor/NR1I2 protein level by ubiquitination and degradation (PubMed:37969738)

Subcellular Location

Nucleus

Tissue Specificity

Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver

Subunit

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1. Interacts with CUL4B and DDB1 (PubMed:25970626). Interacts wwith BRCA1; this interaction mediates BRCA1 ubiquitination and subsequent degradation (PubMed:23086937). Interacts with RGS2; this interaction mediates RGS2 ubiquitination and subsequent degradation (PubMed:25970626). Interacts with PXR/NR1I2; this interaction mediates PXR/NR1I2 ubiquitination and subsequent degradation (PubMed:37969738)

Gene: FBXO44  |  Organism: Homo sapiens  |  Synonyms: F-box protein FBX30; F-box/G-domain protein 3
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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