Recombinant Human Fibronectin (FN1), partial (Active), Homo sapiens (Human), E.coli, Tag-Free, 30.2kDa&32.3kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Fibronectin (FN1), partial (Active)

Recombinant Human Fibronectin (FN1), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >95%.

SKU: BCACP-001108 Category:
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Product SKUBCACP-001108
Product DescriptionRecombinant Human Fibronectin (FN1) protein is expressed from E.coli with Tag-Free. It contains 1270-1546aa & 1721-2016aa. [Accession | P02751].
Uniprot No.P02751
Gene NamesFN1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1270-1546aa & 1721-2016aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight30.2kDa&32.3kDa
ActivityThe esterase activity is determined to be greater than 400 pmol/min/?g.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCIG; Cold insoluble globulin; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; Fibronectin 1; FINC; FINC_HUMAN; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Ugl-Y3

Function

Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix; Secreted
Tissue Specificity: Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine
Disease Association: Glomerulopathy with fibronectin deposits 2 (GFND2) : Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. [The disease is caused by variants affecting the gene represented in this entry] | Spondylometaphyseal dysplasia, corner fracture type (SMDCF) : An autosomal dominant form of spondylometaphyseal dysplasia, a group of short stature disorders distinguished by abnormalities in the vertebrae and the metaphyses of the tubular bones. SMDCF is characterized by flake-like, triangular, or curvilinear ossification centers at the edges of irregular metaphyses that simulate fractures. These corner fractures involve the distal tibia, the ulnar aspect of the distal radius, the proximal humerus, and the proximal femur. They represent irregular ossification at the growth plates and secondary ossification centers. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Fibronectin (FN1), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1270-1546aa & 1721-2016aa, with Tag-Free tag, molecular weight 30.2kDa&32.3kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling

Subcellular Location

Secreted, extracellular space, extracellular matrix; Secreted

Disease Association

Glomerulopathy with fibronectin deposits 2 (GFND2) : Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. [The disease is caused by variants affecting the gene represented in this entry] | Spondylometaphyseal dysplasia, corner fracture type (SMDCF) : An autosomal dominant form of spondylometaphyseal dysplasia, a group of short stature disorders distinguished by abnormalities in the vertebrae and the metaphyses of the tubular bones. SMDCF is characterized by flake-like, triangular, or curvilinear ossification centers at the edges of irregular metaphyses that simulate fractures. These corner fractures involve the distal tibia, the ulnar aspect of the distal radius, the proximal humerus, and the proximal femur. They represent irregular ossification at the growth plates and secondary ossification centers. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) (PubMed:29777959). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine (PubMed:17614963)

Subunit

(Microbial infection) Interacts with fibronectin-binding proteins from other Mycobacteria

Gene: FN1  |  Organism: Homo sapiens  |  Synonyms: Cold-insoluble globulin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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