Recombinant Human Folate receptor alpha (FOLR1), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 25.9kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Folate receptor alpha (FOLR1), partial (Active)

Recombinant Human Folate receptor alpha (FOLR1), partial (Active) — Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Purity >95%.

SKU: BCRECP-000688 Category:
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Product Specifications

Product SkuBCRECP-000688
Product DescriptionRecombinant Human Folate receptor alpha (FOLR1) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 25-233aa. [Accession | P15328].
Uniprot No.P15328
Gene NamesFOLR1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region25-233aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight25.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor); Apical cell membrane (Lipid-anchor, GPI-anchor); Basolateral cell membrane (Lipid-anchor, GPI-like-anchor); Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome
Tissue Specificity: Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium
Disease Association: Neurodegeneration due to cerebral folate transport deficiency (NCFTD) : An autosomal recessive neurodegenerative disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy and leukodystrophy. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Folate receptor alpha (FOLR1), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 25-233aa, with C-terminal 10xHis-tagged tag, molecular weight 25.9kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor); Apical cell membrane (Lipid-anchor, GPI-anchor); Basolateral cell membrane (Lipid-anchor, GPI-like-anchor); Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome

Disease Association

Neurodegeneration due to cerebral folate transport deficiency (NCFTD) : An autosomal recessive neurodegenerative disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy and leukodystrophy. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium

Gene: FOLR1  |  Organism: Homo sapiens  |  Synonyms: Adult folate-binding protein; Folate receptor 1; Folate receptor, adult; KB cells FBP; Ovarian tumor-associated antigen MOv18
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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