Recombinant Human Galectin-9 (LGALS9) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 42.2 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Galectin-9 (LGALS9) (Active)

Recombinant Human Galectin-9 (LGALS9) (Active) — Acts as an eosinophil chemoattractant. Purity >95%.

SKU: BCACP-000626 Category:
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Product SKUBCACP-000626
Product DescriptionRecombinant Human Galectin-9 (LGALS9) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1-355aa. [Accession | O00182].
Uniprot No.O00182
Gene NamesLGALS9
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1-355aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight42.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human HSPA5 at 2 μg/mL can bind Anti-HSPA5 recombinant antibody . The EC50 is 3.767-4.368 ng/mL.;HSPA5 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human HSPA5 with an affinity constant of 18 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesGalectin-9; Gal-9; Ecalectin; Tumor antigen HOM-HD-21

Function

Acts as an eosinophil chemoattractant. It also inhibits angiogenesis. Suppresses IFNG production by natural killer cells.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Peripheral blood leukocytes and lymphatic tissues. Expressed in lung, liver, breast and kidney with higher levels in tumor endothelial cells than normal endothelium (at protein level). Expressed in trophoblast cells in decidua and placenta in pregnancy (at protein level). Isoform 2 is the most abundant isoform expressed in endothelial cells. Upon endothelial cell activation isoform 2 expression decreases while expression of isoform 3 and isoform 5 increases. Isoform 4 decreases in pathological pregnancy

Product Specifications

Recombinant Human Galectin-9 (LGALS9) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-355aa, with C-terminal 10xHis-tagged tag, molecular weight 42.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as an eosinophil chemoattractant (PubMed:9642261). It also inhibits angiogenesis (PubMed:24333696). Suppresses IFNG production by natural killer cells (By similarity)

Subcellular Location

Secreted

Tissue Specificity

Peripheral blood leukocytes and lymphatic tissues. Expressed in lung, liver, breast and kidney with higher levels in tumor endothelial cells than normal endothelium (at protein level) (PubMed:24333696). Expressed in trophoblast cells in decidua and placenta in pregnancy (at protein level) (PubMed:23242525, PubMed:25578313). Isoform 2 is the most abundant isoform expressed in endothelial cells (PubMed:24333696). Upon endothelial cell activation isoform 2 expression decreases while expression of isoform 3 and isoform 5 increases (PubMed:24333696). Isoform 4 decreases in pathological pregnancy (PubMed:23242525)

Subunit

Monomer

Gene: LGALS9  |  Organism: Homo sapiens  |  Synonyms: Ecalectin; Tumor antigen HOM-HD-21
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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