Recombinant Human Gamma-interferon-inducible lysosomal thiol reductase (IFI30), partial

Function

Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction (PubMed:10639150). Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation (PubMed:10639150). Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction (PubMed:10852914). Dithiothreitol, cysteine, and cysteinyl glycine, but not glutathione, are capable of regenerating active precursor and mature IFI30 in vitro (PubMed:10639150, PubMed:10852914)

Subcellular Location

Secreted; Lysosome

Tissue Specificity

Expressed constitutively in antigen-presenting cells

Subunit

Dimer; disulfide-linked

• Luster A.D. et al. Molecular and biochemical characterization of a novel gamma-interferon-inducible protein. — J. Biol. Chem. (1988)
• Arunachalam B. et al. Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). — Proc. Natl. Acad. Sci. U.S.A. (2000)
• Arahira M. et al. A novel Asn-bond specific endoproteolytic enzyme from Human. (OCT-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Grimwood J. et al. The DNA sequence and biology of human chromosome 19. — Nature (2004)
• Mural R.J. et al. PubMed: CI-5GBDQ6B103N1E (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Phan U.T. et al. Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. — J. Biol. Chem. (2000)