Recombinant Human Gap junction alpha-5 protein (GJA5), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 21.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Gap junction alpha-5 protein (GJA5), partial

Recombinant Human Gap junction alpha-5 protein (GJA5), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-000749 Category:
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Product Specifications

Product SkuBCRECP-000749
Product DescriptionRecombinant Human Gap junction alpha-5 protein (GJA5) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 227-358aa. [Accession | P36382].
Uniprot No.P36382
Gene NamesGJA5
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region227-358aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight21.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell junction, gap junction
Disease Association: Atrial standstill 1 (ATRST1) : A rare arrhythmia characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. [The disease may be caused by variants affecting distinct genetic loci, including the gene represented in this entry. A rare GJA5 genotype has been detected in combination with a mutation in SCN5A in a large family with atrial standstill] | Atrial fibrillation, familial, 11 (ATFB11) : A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Gap junction alpha-5 protein (GJA5), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 227-358aa, with C-terminal 6xHis-tagged tag, molecular weight 21.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell junction, gap junction

Disease Association

Atrial standstill 1 (ATRST1) : A rare arrhythmia characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. [The disease may be caused by variants affecting distinct genetic loci, including the gene represented in this entry. A rare GJA5 genotype has been detected in combination with a mutation in SCN5A in a large family with atrial standstill] | Atrial fibrillation, familial, 11 (ATFB11) : A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

A connexon is composed of a hexamer of connexins

Gene: GJA5  |  Organism: Homo sapiens  |  Synonyms: Connexin-40
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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