Recombinant Human Golgi phosphoprotein 3 (GOLPH3), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 39.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Golgi phosphoprotein 3 (GOLPH3)

Recombinant Human Golgi phosphoprotein 3 (GOLPH3) — Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Purity >85%.

SKU: BCRECP-000769 Category:
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Product Specifications

Product SkuBCRECP-000769
Product DescriptionRecombinant Human Golgi phosphoprotein 3 (GOLPH3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-298aa. [Accession | Q9H4A6].
Uniprot No.Q9H4A6
Gene NamesGOLPH3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-298aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight39.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking.

Biological Context

Subcellular Location: Golgi apparatus, Golgi stack membrane (Peripheral membrane protein); Golgi apparatus, trans-Golgi network membrane (Peripheral membrane protein); Mitochondrion intermembrane space; Cell membrane; Endosome
Tissue Specificity: Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers

Product Specifications

Recombinant Human Golgi phosphoprotein 3 (GOLPH3) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-298aa, with N-terminal 6xHis-tagged tag, molecular weight 39.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis

Subcellular Location

Golgi apparatus, Golgi stack membrane (Peripheral membrane protein); Golgi apparatus, trans-Golgi network membrane (Peripheral membrane protein); Mitochondrion intermembrane space; Cell membrane; Endosome

Tissue Specificity

Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers

Subunit

Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35

Gene: GOLPH3  |  Organism: Homo sapiens  |  Synonyms: Coat protein GPP34; Mitochondrial DNA absence factor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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