Recombinant Human Helicase POLQ-like (HELQ), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 49.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Helicase POLQ-like (HELQ), partial

Recombinant Human Helicase POLQ-like (HELQ), partial — Single-stranded 3′-5′ DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Purity >90%.

SKU: BCRECP-000815 Category:
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Product Specifications

Product SkuBCRECP-000815
Product DescriptionRecombinant Human Helicase POLQ-like (HELQ) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 350-726aa. [Accession | Q8TDG4].
Uniprot No.Q8TDG4
Gene NamesHELQ
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region350-726aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight49.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA). Possesses both DNA unwinding and annealing activities.

Biological Context

Subcellular Location: Nucleus; Chromosome

Product Specifications

Recombinant Human Helicase POLQ-like (HELQ), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 350-726aa, with C-terminal 6xHis-tagged tag, molecular weight 49.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair (PubMed:11751861, PubMed:19995904, PubMed:21398521, PubMed:24005041, PubMed:24005565, PubMed:34316696, PubMed:34937945). Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA) (PubMed:34937945). Possesses both DNA unwinding and annealing activities (PubMed:34937945). Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA (PubMed:34937945). Efficiently unwinds substrates containing 3' overhangs or a D-loop (PubMed:21398521, PubMed:34937945). In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing (PubMed:34937945). Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences (PubMed:34316696, PubMed:34937945)

Subcellular Location

Nucleus; Chromosome

Subunit

Homodimer (PubMed:34316696). Interacts with POLN (PubMed:19995904). Interacts with RAD51B and RAD51C; promoting association with the BCDX2 complex (PubMed:24005041). Interacts with the replication protein A (RPA/RP-A) complex (PubMed:34316696, PubMed:34937945). Interacts with RAD51; stimulating HELQ DNA helicase activity and ability to unwing DNA (PubMed:34937945)

Gene: HELQ  |  Organism: Homo sapiens  |  Synonyms: Mus308-like helicase; POLQ-like helicase
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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