Recombinant Human Holliday junction recognition protein (HJURP)

Function

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions

Subcellular Location

Nucleus, nucleolus; Chromosome, centromere

Tissue Specificity

According to PubMed:17256767, highly expressed in the thymus with lower levels in the placenta, small intestine, liver, skeletal muscle, and colon. According to PubMed:17823411, highly expressed in testis, and at a relatively lower level in thymus and bone marrow. Significantly overexpressed in many lung cancer samples, compared with normal lung

Subunit

Interacts with CENPA (via CATD domain); the interaction is direct and specific for CENPA since it does not interact with H3.1- or H3.3-containing nucleosomes (PubMed:16622419, PubMed:19410544, PubMed:19410545). Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4 (PubMed:21478274). Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292). Interacts with 14-3-3 family members in a phosphorylation-dependent manner (PubMed:17256767). Interacts with MSH5 and NBN (PubMed:17823411)

• Kato T. et al. Activation of Holliday junction recognizing protein involved in the chromosomal stability and immortality of cancer cells. — Cancer Res. (2007)
• Koike N. et al. Impaired cytoplasmic localization and nuclear accumulation of a novel gene product, hFLEG1, associated with hepatocellular carcinoma development. (FEB-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Hillier L.W. et al. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. — Nature (2005)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Bechtel S. et al. The full-ORF clone resource of the German cDNA consortium. — BMC Genomics (2007)
• Beausoleil S.A. et al. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. — Nat. Biotechnol. (2006)
• Foltz D.R. et al. The human CENP-A centromeric nucleosome-associated complex. — Nat. Cell Biol. (2006)