Recombinant Human Hyaluronidase PH-20 (SPAM1), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 67.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Hyaluronidase PH-20 (SPAM1)

Recombinant Human Hyaluronidase PH-20 (SPAM1) — Involved in sperm-egg adhesion. Purity >90%.

SKU: BCRECP-001687 Category:
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Product Specifications

Product SkuBCRECP-001687
Product DescriptionRecombinant Human Hyaluronidase PH-20 (SPAM1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 36-490aa. [Accession | P38567].
Uniprot No.P38567
Gene NamesSPAM1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region36-490aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight67.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)
Tissue Specificity: Testis

Product Specifications

Recombinant Human Hyaluronidase PH-20 (SPAM1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 36-490aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 67.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor)

Tissue Specificity

Testis

Gene: SPAM1  |  Organism: Homo sapiens  |  Synonyms: Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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