Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), Homo sapiens (Human), Yeast, C-terminal GST-tagged, 65.8kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN)

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) — Hydroxylates HIF-1 alpha at ‘Asn-803’ in the C-terminal transactivation domain (CAD). Purity >95%.

SKU: BCRECP-000819 Category:
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Product Specifications

Product SkuBCRECP-000819
Product DescriptionRecombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) Protein is expressed from Yeast with C-terminal GST-tagged. It contains 2-349aa. [Accession | Q9NWT6].
Uniprot No.Q9NWT6
Gene NamesHIF1AN
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-349aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal GST-tagged
Molecular weight65.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Biological Context

Subcellular Location: Nucleus; Cytoplasm; Cytoplasm, perinuclear region

Product Specifications

Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 2-349aa, with C-terminal GST-tagged tag, molecular weight 65.8kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation

Subcellular Location

Nucleus; Cytoplasm; Cytoplasm, perinuclear region

Subunit

Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4 (By similarity). Interacts with UBE3A. Interacts with ANKS3 (By similarity). Interacts with NECAB3; the interaction is indirect and seems to be mediated by APBA3

Gene: HIF1AN  |  Organism: Homo sapiens  |  Synonyms: Factor inhibiting HIF-1; Hypoxia-inducible factor asparagine hydroxylase
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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