Recombinant Human Inactive tyrosine-protein kinase 7(PTK7),partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 76.0 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
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Recombinant Human Inactive tyrosine-protein kinase 7(PTK7),partial (Active)

Recombinant Human Inactive tyrosine-protein kinase 7(PTK7),partial (Active) — Inactive tyrosine kinase involved in the Wnt signaling pathway. Purity >95%.

SKU: BCACP-000307 Categories: , ,
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Product SKUBCACP-000307
Product DescriptionRecombinant Human Inactive tyrosine-protein kinase 7(PTK7) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 31-704aa. [Accession | Q13308].
Uniprot No.Q13308
Gene NamesPTK7
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region31-704aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight76.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 5 μg/mL on an Nickel Coated plate can bind Anti-CLDN18.2 recombinant antibody , the EC50 is 0.4640-0.6372 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesColon carcinoma kinase 4 (CCK-4); Protein-tyrosine kinase 7;Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7

Function

Inactive tyrosine kinase involved in the Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathways. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis.

Product Specifications

Recombinant Human Inactive tyrosine-protein kinase 7(PTK7),partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 31-704aa, with C-terminal 10xHis-tagged tag, molecular weight 76.0 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inactive tyrosine kinase involved in the Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathways. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Does not bind ATP (PubMed:24107129, PubMed:32619402)

Gene: PTK7  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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