Recombinant Human Interleukin-3 (IL3) (Active), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 22.0 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Interleukin-3 (IL3) (Active)

Recombinant Human Interleukin-3 (IL3) (Active) — Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into linea… Purity >95%.

SKU: BCACP-000039 Categories: ,
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Product SKUBCACP-000039
Product DescriptionRecombinant Human Interleukin-3 (IL3) protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 20-152aa. [Accession | P08700].
Uniprot No.P08700
Gene NamesIL3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region20-152aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight22.0 kDa
ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 5.972-17.16 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesInterleukin-3; IL-3;Hematopoietic growth factor; Mast cell growth factor (MCGF); Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3

Function

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Activated T-cells, mast cells, natural killer cells

Product Specifications

Recombinant Human Interleukin-3 (IL3) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 20-152aa, with C-terminal 6xHis-tagged tag, molecular weight 22.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 5.972-17.16 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed:2556442). Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated (PubMed:10779277, PubMed:32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed:23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed:29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed:27862234)

Subcellular Location

Secreted

Tissue Specificity

Activated T-cells, mast cells, natural killer cells

Subunit

Interacts with IL3RA (PubMed:29374162)

Gene: IL3  |  Organism: Homo sapiens  |  Synonyms: Hematopoietic growth factor; Mast cell growth factor; Multipotential colony-stimulating factor; P-cell-stimulating factor
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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