Recombinant Human Kallikrein-2 (KLK2) (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >90%.
| Product SKU | BCACP-000624 |
| Product Description | Recombinant Human Kallikrein-2 (KLK2) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-261aa. [Accession | P20151]. |
| Uniprot No. | P20151 |
| Gene Names | KLK2 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 19-261aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 29.6 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human KLK2 at 2 u03bcg/mL can bind Anti-KLK2 recombinant antibody. The EC50 is 0.6741-0.8245 ng/mL.;Measured by its ability to cleave a fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >350 pmol/min/u03bcg. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Kallikrein-2; EC 3.4.21.35; Glandular kallikrein-1; hGK-1; Tissue kallikrein-2 |
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Recombinant Human Kallikrein-2 (KLK2) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-261aa, with C-terminal 10xHis-tagged tag, molecular weight 29.6 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KLK2 at 2 u03bcg/mL can bind Anti-KLK2 recombinant antibody. The EC50 is 0.6741-0.8245 ng/mL.;Measured by its ability to cleave a fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >350 pmol/min/u03bcg.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin
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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.
Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
