Recombinant Human Legumain (LGMN) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 49.0 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Legumain (LGMN) (Active)

Recombinant Human Legumain (LGMN) (Active) — Has a strict specificity for hydrolysis of asparaginyl bonds. Purity >95%.

SKU: BCACP-000591 Category:
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Product SKUBCACP-000591
Product DescriptionRecombinant Human Legumain (LGMN) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 18-433aa. [Accession | Q99538].
Uniprot No.Q99538
Gene NamesLGMN
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region18-433aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight49.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human LGALS3 at 2 μg/mL can bind anti-LGALS3 recombinant antibody.The EC50 is 2.361-2.785 ng/mL.;LGALS3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human LGALS3 with an affinity constant of 7.54 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesLegumain; EC 3.4.22.34; Asparaginyl endopeptidase; AEP; Protease, cysteine 1

Function

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Biological Context

Subcellular Location: Lysosome
Tissue Specificity: Ubiquitous. Particularly abundant in kidney, heart and placenta

Product Specifications

Recombinant Human Legumain (LGMN) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 18-433aa, with C-terminal 10xHis-tagged tag, molecular weight 49.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LGALS3 at 2 μg/mL can bind anti-LGALS3 recombinant antibody.The EC50 is 2.361-2.785 ng/mL.;LGALS3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human LGALS3 with an affinity constant of 7.54 nM as detected by MetaSPR Assay (WeSPRTM 200).

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed:9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity)

Subcellular Location

Lysosome

Tissue Specificity

Ubiquitous. Particularly abundant in kidney, heart and placenta

Subunit

Homodimer before autocatalytic removal of the propeptide (PubMed:23776206). Monomer after autocatalytic processing (PubMed:23776206). May interact with integrins (PubMed:23776206)

Gene: LGMN  |  Organism: Homo sapiens  |  Synonyms: Asparaginyl endopeptidase; Protease, cysteine 1
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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