Recombinant Human Mas-related G-protein coupled receptor member D (MRGPRD) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 42.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Mas-related G-protein coupled receptor member D (MRGPRD) Protein

Recombinant Human Mas-related G-protein coupled receptor member D (MRGPRD) Protein — G protein-coupled receptor that acts as a mediator of peripheral pain and itch sensations. Purity >85%.

SKU: BCRECP-00191TM Category:
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Product Specifications

Uniprot No.Q8TDS7
Gene NamesMRGPRD
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-321aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight42.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor that acts as a mediator of peripheral pain and itch sensations. Activated by various ligands, such as beta-alanine, beta-aminoisobutyrate, angiotensin 1-7, alamandine and allantoin, causing a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. MRGPRD is both coupled to G(q) and G(i) G proteins: G(q) coupling activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers, while G(i) coupling mediates inhibition of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Human Mas-related G-protein coupled receptor member D (MRGPRD) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-321aa, with N-terminal 10xHis-tagged tag, molecular weight 42.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

G protein-coupled receptor that acts as a mediator of peripheral pain and itch sensations (PubMed:15037633, PubMed:23091359, PubMed:35840655, PubMed:39580805). Activated by various ligands, such as beta-alanine, beta-aminoisobutyrate, angiotensin 1-7, alamandine and allantoin, causing a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:15037633, PubMed:23091359, PubMed:35840655, PubMed:39580805). MRGPRD is both coupled to G(q) and G(i) G proteins: G(q) coupling activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers, while G(i) coupling mediates inhibition of adenylate cyclase activity (PubMed:15037633, PubMed:23091359, PubMed:35840655, PubMed:39580805). MRGPRD is specifically expressed in neurons that innervate the epidermis and acts as a key mediator of skin stimuli, such as itch, pain and mechanical stimuli (By similarity). Required to maintain skin homeostasis in response to irritant dermatitis by initiating a signaling that promotes glutamate release, inhibiting mast cell hyperresponsiveness and skin inflammation (By similarity). Required to prevent cardiomyocyte hypertrophy following activation by alamandine, a decarboxylation product of angiotensin 1-7 (By similarity). Acts as a receptor for allantoin in dorsal root ganglion neurons, eliciting chronic itch (pruritus) (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Gene: MRGPRD  |  Organism: Homo sapiens  |  Synonyms: Beta-alanine receptor; G protein-coupled receptor TGR7
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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