Recombinant Human Monocyte differentiation antigen CD14 (CD14), Homo sapiens (Human), Mammalian cell, N-terminal 6xHis-Myc-tagged, 39.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Monocyte differentiation antigen CD14 (CD14)

Recombinant Human Monocyte differentiation antigen CD14 (CD14) — Coreceptor for bacterial lipopolysaccharide. Purity >90%.

SKU: BCRECP-000261 Categories: ,
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Product Specifications

Product SkuBCRECP-000261
Product DescriptionRecombinant Human Monocyte differentiation antigen CD14 (CD14) Protein is expressed from Mammalian cell with N-terminal 6xHis-Myc-tagged. It contains 20-345aa. [Accession | P08571].
Uniprot No.P08571
Gene NamesCD14
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region20-345aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-Myc-tagged
Molecular weight39.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor); Secreted; Membrane raft; Golgi apparatus
Tissue Specificity: Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages

Product Specifications

Recombinant Human Monocyte differentiation antigen CD14 (CD14) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 20-345aa, with N-terminal 6xHis-Myc-tagged tag, molecular weight 39.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor); Secreted; Membrane raft; Golgi apparatus

Tissue Specificity

Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages

Subunit

Interacts with LPS-bound LPB (PubMed:1698311, PubMed:23264655). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (PubMed:16880211). Interacts with MYO18A (PubMed:25965346). Interacts with FSTL1 (PubMed:22265692)

Gene: CD14  |  Organism: Homo sapiens  |  Synonyms: My23 antigen; Myeloid cell-specific leucine-rich glycoprotein
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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