Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 26.7kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active)

Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active) — Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Purity >95%.

SKU: BCRECP-001210 Category:
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Product Specifications

Product SkuBCRECP-001210
Product DescriptionRecombinant Human Myosin regulatory light chain 12B (MYL12B) (Active) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-172aa. [Accession | O14950].
Uniprot No.O14950
Gene NamesMYL12B
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-172aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight26.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

Biological Context

Tissue Specificity: Ubiquitously expressed in various hematopoietic cells

Product Specifications

Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-172aa, with C-terminal 6xHis-tagged tag, molecular weight 26.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion

Tissue Specificity

Ubiquitously expressed in various hematopoietic cells

Subunit

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19

Gene: MYL12B  |  Organism: Homo sapiens  |  Synonyms: MLC-2A; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain MRLC2; SHUJUN-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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