Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2), Homo sapiens (Human), E.coli, N-terminal 6xHis-GST-tagged, 61.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2)

Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2) — Putative hydrolase with unknown substrate (Probable). Purity >85%.

SKU: BCRECP-000486 Categories: ,
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Product Specifications

Product SkuBCRECP-000486
Product DescriptionRecombinant Human N (G) Protein is expressed from E.coli with N-terminal 6xHis-GST-tagged. It contains 1-285aa. [Accession | O95865].
Uniprot No.O95865
Gene NamesDDAH2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-285aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-GST-tagged
Molecular weight61.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS. In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase.

Biological Context

Subcellular Location: Mitochondrion
Tissue Specificity: Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain

Product Specifications

Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-285aa, with N-terminal 6xHis-GST-tagged tag, molecular weight 61.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed:21493890, PubMed:37296100). In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase (By similarity). Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein (By similarity). Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS (PubMed:33850055)

Subcellular Location

Mitochondrion

Tissue Specificity

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain

Gene: DDAH2  |  Organism: Homo sapiens  |  Synonyms: DDAHII; Inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; Protein G6a; S-phase protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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