Recombinant Human NADP-dependent malic enzyme, mitochondrial (ME3), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 67.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human NADP-dependent malic enzyme, mitochondrial (ME3), partial

Recombinant Human NADP-dependent malic enzyme, mitochondrial (ME3), partial — Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+), and is able to reverse the reaction but only with significantly lower efficiency. Purity >85%.

SKU: BCRECP-001143 Categories: ,
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Product Specifications

Product SkuBCRECP-001143
Product DescriptionRecombinant Human NADP-dependent malic enzyme, mitochondrial (ME3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 24-590aa. [Accession | Q16798].
Uniprot No.Q16798
Gene NamesME3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-590aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight67.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+), and is able to reverse the reaction but only with significantly lower efficiency. Can also catalyze the decarboxylation of oxaloacetate into pyruvate.

Biological Context

Subcellular Location: Mitochondrion matrix
Tissue Specificity: Expressed predominantly in organs with a low-division rate

Product Specifications

Recombinant Human NADP-dependent malic enzyme, mitochondrial (ME3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 24-590aa, with N-terminal 6xHis-tagged tag, molecular weight 67.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+), and is able to reverse the reaction but only with significantly lower efficiency (PubMed:7818469). Can also catalyze the decarboxylation of oxaloacetate into pyruvate (By similarity)

Subcellular Location

Mitochondrion matrix

Tissue Specificity

Expressed predominantly in organs with a low-division rate

Subunit

Homotetramer

Gene: ME3  |  Organism: Homo sapiens  |  Synonyms: Malic enzyme 3

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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