Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 42.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial — Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Purity >85%.

SKU: BCRECP-001242 Category:
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Product Specifications

Product SkuBCRECP-001242
Product DescriptionRecombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A). [Accession | P14543].
Uniprot No.P14543
Gene NamesNID1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A)
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight42.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane

Product Specifications

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A), with C-terminal 6xHis-tagged tag, molecular weight 42.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane

Subunit

Interacts with FBLN1 (By similarity). Interacts with LGALS3BP (By similarity). Interacts with PLXDC1 (PubMed:16574105). Interacts with SVEP1 (By similarity)

Gene: NID1  |  Organism: Homo sapiens  |  Synonyms: Entactin
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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