Recombinant Human Nitric oxide synthase, inducible (NOS2), partial

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109)

Subcellular Location

Cytoplasm, cytosol

Tissue Specificity

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305)

Subunit

Homodimer (PubMed:10074942, PubMed:10409685). Interacts with NHERF1 (PubMed:12080081). Interacts with GAPDH; induced by oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:25417112). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (PubMed:25417112). Interacts with SPSB1, SPSB2 and SPSB4 (PubMed:21199876). Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4 (PubMed:21199876). Forms a complex with ASL, ASS1 and HSP90AA1; the complex regulates cell-autonomous L-arginine synthesis and citrulline recycling while channeling extracellular L-arginine to nitric oxide synthesis pathway

• Sherman P.A. et al. Purification and cDNA sequence of an inducible nitric oxide synthase from a human tumor cell line. — Biochemistry (1993)
• Geller D.A. et al. Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. — Proc. Natl. Acad. Sci. U.S.A. (1993)
• Charles I.G. et al. Cloning, characterization, and expression of a cDNA encoding an inducible nitric oxide synthase from the human chondrocyte. — Proc. Natl. Acad. Sci. U.S.A. (1993)
• Maier R. et al. Inducible nitric oxide synthase from human articular chondrocytes: cDNA cloning and analysis of mRNA expression. — Biochim. Biophys. Acta (1994)
• Park C.S. et al. Human retina expresses both constitutive and inducible isoforms of nitric oxide synthase mRNA. — Biochem. Biophys. Res. Commun. (1994)
• Hokari A. et al. Cloning and functional expression of human inducible nitric oxide synthase (NOS) cDNA from a glioblastoma cell line A-172. — J. Biochem. (1994)
• Guo F.H. et al. Continuous nitric oxide synthesis by inducible nitric oxide synthase in normal human airway epithelium in vivo. — Proc. Natl. Acad. Sci. U.S.A. (1995)
• Luss H. et al. Dedifferentiated human ventricular cardiac myocytes express inducible nitric oxide synthase mRNA but not protein in response to IL-1, TNF, IFNgamma, and LPS. — J. Mol. Cell. Cardiol. (1997)