Recombinant Human Non-structural maintenance of chromosomes element 3 homolog (NSMCE3), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 38.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Non-structural maintenance of chromosomes element 3 homolog (NSMCE3)

Recombinant Human Non-structural maintenance of chromosomes element 3 homolog (NSMCE3) — Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. Purity >85%.

SKU: BCRECP-001282 Category:
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Product Specifications

Product SkuBCRECP-001282
Product DescriptionRecombinant Human Non-structural maintenance of chromosomes element 3 homolog (NSMCE3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-304aa. [Accession | Q96MG7].
Uniprot No.Q96MG7
Gene NamesNSMCE3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-304aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight38.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Product Specifications

Recombinant Human Non-structural maintenance of chromosomes element 3 homolog (NSMCE3) — Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination (PubMed:20864041, PubMed:27427983). The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:20864041). May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest (By similarity)

Subcellular Location

Cytoplasm; Nucleus; Chromosome, telomere

Disease Association

Lung disease, immunodeficiency, and chromosome breakage syndrome (LICS) : An autosomal recessive chromosome breakage syndrome associated with severe, fatal lung disease in early childhood, following viral pneumonia. LICS is characterized by combined T and B-cell immunodeficiency. Some patients may have mild dysmorphic features. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Ubiquitous

Subunit

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. NSMCE1, NSMCE4A or EID3 and NSMCE3 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer (PubMed:18086888). Interacts with PJA1 (PubMed:20864041). Interacts with E2F1 (via C-terminus) (By similarity). Interacts with NGFR (via C-terminus) (By similarity). Interacts with NSMCE1 (PubMed:18086888, PubMed:20864041, PubMed:21364888, PubMed:27427983). Interacts with NSMCE4 (PubMed:20864041, PubMed:27427983). Interacts with SMC6 (PubMed:18086888). Interacts with EID3 (PubMed:21364888)

Gene: NSMCE3  |  Organism: Homo sapiens  |  Synonyms: Hepatocellular carcinoma-associated protein 4; MAGE-G1 antigen; Melanoma-associated antigen G1; Necdin-like protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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