Recombinant Human Oncostatin-M (OSM), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 24.4 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Oncostatin-M (OSM), partial (Active)

Recombinant Human Oncostatin-M (OSM), partial (Active) — Functions as a cytokine that uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Purity >95%.

SKU: BCACP-000010 Category:
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Product SKUBCACP-000010
Product DescriptionRecombinant Human Oncostatin-M (OSM) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 26-221aa. [Accession | P13725].
Uniprot No.P13725
Gene NamesOSM
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region26-221aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight24.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human OSM at 2 μg/mL can bind Anti-OSM recombinant antibody , the EC50 is 3.048-3.860 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Functions as a cytokine that uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Functionally, regulates many processes including cell proliferation, cell differentiation, cytokine production. Mechanistically, low affinity ligand binding to IL6ST/gp130, induces heterodimerization with LIFR or OSMR, activating JAK tyrosine kinases (JAK1 or JAK2 and to a lesser extent TYK2) bound to their intracellular domains.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Human Oncostatin-M (OSM), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 26-221aa, with C-terminal 6xHis-tagged tag, molecular weight 24.4 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Functions as a cytokine that uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST) (PubMed:10997905, PubMed:39532904, PubMed:8999038). Functionally, regulates many processes including cell proliferation, cell differentiation, cytokine production (PubMed:10997905, PubMed:1542792, PubMed:1542793, PubMed:1717982, PubMed:2779549, PubMed:3540948). Mechanistically, low affinity ligand binding to IL6ST/gp130, induces heterodimerization with LIFR or OSMR, activating JAK tyrosine kinases (JAK1 or JAK2 and to a lesser extent TYK2) bound to their intracellular domains (PubMed:9188471). These kinases subsequently phosphorylate IL6ST/gp130 and LIFR or OSMR (PubMed:8999038). The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of STAT3 (PubMed:9188471). The type II OSM complex receptor is also able in addition to STAT3 to recruit STAT5B (PubMed:9188471). Moreover, the type II OSM complex receptor recruits SHC1 through OSMR in a JAK1 mediated phosphotyrosine-dependent manner, leading to SHC1 association with GRB2 and downstream activation of the Ras/Raf/MAPK pathway (PubMed:11016927)

Subcellular Location

Secreted

Gene: OSM  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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