Recombinant Human Osteopontin (SPP1) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 35.1 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Osteopontin (SPP1) (Active)

Recombinant Human Osteopontin (SPP1) (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000583 Category:
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Product SKUBCACP-000583
Product DescriptionRecombinant Human Osteopontin (SPP1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 17-314aa. [Accession | P10451].
Uniprot No.P10451
Gene NamesSPP1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region17-314aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight35.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.2007-0.2567 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.3261-0.3657 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesOsteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

Function

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma

Product Specifications

Recombinant Human Osteopontin (SPP1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 17-314aa, with C-terminal 10xHis-tagged tag, molecular weight 35.1 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.2007-0.2567 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.3261-0.3657 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Subcellular Location

Secreted

Tissue Specificity

Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717). Bone. Found in plasma

Subunit

Interacts (via N-terminus) with integrin ITGA9:ITGB1

Gene: SPP1  |  Organism: Homo sapiens  |  Synonyms: Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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