Recombinant Human Osteopontin (SPP1) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 35.1 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Osteopontin (SPP1) (Active)

Recombinant Human Osteopontin (SPP1) (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000583 Category:
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Product SKUBCACP-000583
Product DescriptionRecombinant Human Osteopontin (SPP1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 17-314aa. [Accession | P10451].
Uniprot No.P10451
Gene NamesSPP1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region17-314aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight35.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.2007-0.2567 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 0.3261-0.3657 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesOsteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

Function

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma

Product Specifications

Recombinant Human Osteopontin (SPP1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 17-314aa, with C-terminal 10xHis-tagged tag, molecular weight 35.1 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Subcellular Location

Secreted

Tissue Specificity

Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717). Bone. Found in plasma

Subunit

Interacts (via N-terminus) with integrin ITGA9:ITGB1

Gene: SPP1  |  Organism: Homo sapiens  |  Synonyms: Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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