Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active), Human papillomavirus type 16, E.coli, N-terminal 6xHis-tagged and C-terminal 6xHis-tagged, 16.3 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active)

Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active) — Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Purity >90%.

SKU: BCACP-000144 Categories: , , ,
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Product SKUBCACP-000144
Product DescriptionRecombinant Human papillomavirus type 16 Protein E7 (E7) protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged. It contains 1-98aa. [Accession | P03129].
Uniprot No.P03129
Gene NamesE7
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-98aa
SpeciesHuman papillomavirus type 16
Tag InfoN-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Molecular weight16.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ROR1 at 2 μg/mL can bind anti-ROR1 antibody（CSB-RA020067A1HU）, the EC50 is 0.2450-0.3416 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesProtein E7

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities.

Product Specifications

Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active) is a recombinant protein, expressed in E.coli, covering amino acids 1-98aa, with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged tag, molecular weight 16.3 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta)

Gene: E7  |  Organism: Human papillomavirus type 16

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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