Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active), Human papillomavirus type 16, E.coli, N-terminal 6xHis-tagged and C-terminal 6xHis-tagged, 16.3 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active)

Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active) — Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Purity >90%.

SKU: BCACP-000144 Categories: , ,
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Product SKUBCACP-000144
Product DescriptionRecombinant Human papillomavirus type 16 Protein E7 (E7) protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged. It contains 1-98aa. [Accession | P03129].
Uniprot No.P03129
Gene NamesE7
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-98aa
SpeciesHuman papillomavirus type 16
Tag InfoN-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Molecular weight16.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized ROR1 at 2 μg/mL can bind anti-ROR1 antibody（CSB-RA020067A1HU）, the EC50 is 0.2450-0.3416 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesProtein E7

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities.

Product Specifications

Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active) is a recombinant protein, expressed in E.coli, covering amino acids 1-98aa, with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged tag, molecular weight 16.3 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized ROR1 at 2 μg/mL can bind anti-ROR1 antibody（CSB-RA020067A1HU）, the EC50 is 0.2450-0.3416 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta)

Gene: E7  |  Organism: Human papillomavirus type 16

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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