Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial, Human parvovirus B19 (isolate AU) (HPV B19), E.coli, N-terminal 6xHis-SUMO-tagged, 44.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial

Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial — Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promo… Purity >90%.

SKU: BCRECP-003912 Categories: ,
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Product Specifications

Product SkuBCRECP-003912
Product DescriptionRecombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-255aa. [Accession | P07298].
Uniprot No.P07298
Gene NamesNS1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-255aa
SpeciesHuman parvovirus B19 (isolate AU) (HPV B19)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight44.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters.

Biological Context

Subcellular Location: Host nucleus

Product Specifications

Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial is a recombinant protein from Human parvovirus B19 (isolate AU) (HPV B19), expressed in E.coli, covering amino acids 1-255aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 44.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters. Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins. Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition. This arrest promotes apoptosis for viral release

Subcellular Location

Host nucleus

Subunit

Homooligomer; when bound to DNA. Interacts with host E2F4 and E2F5; these interactions promote the nuclear import of E2F4 and E2F5 and the G2/M block observed in host cells. Interacts with host transcription factor SP1; this interaction is important for NS1 transcriptional regulation of p21/WAF1. Interacts with host transcription factor SP3

Gene: NS1  |  Organism: Human parvovirus B19 (isolate AU)  |  Synonyms: NCVP1; Non-capsid protein NS-1; Non-structural protein 1; Non-structural protein NS1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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