Recombinant Human Potassium-transporting ATPase subunit beta (ATP4B), partial, Homo sapiens (Human), E.coli, Tag-Free, 26.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Potassium-transporting ATPase subunit beta (ATP4B), partial

Recombinant Human Potassium-transporting ATPase subunit beta (ATP4B), partial — The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Purity >85%.

SKU: BCRECP-000130 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-000130
Product DescriptionRecombinant Human Potassium-transporting ATPase subunit beta (ATP4B) Protein is expressed from E.coli with Tag-Free. It contains 58-291aa. [Accession | P51164].
Uniprot No.P51164
Gene NamesATP4B
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region58-291aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight26.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2).

Biological Context

Subcellular Location: Apical cell membrane (Single-pass type II membrane protein); Cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Human Potassium-transporting ATPase subunit beta (ATP4B), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 58-291aa, with Tag-Free tag, molecular weight 26.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity). Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity)

Subcellular Location

Apical cell membrane (Single-pass type II membrane protein); Cell membrane (Single-pass type II membrane protein)

Subunit

The ATPase pump is composed of two subunits: alpha (catalytic) and beta (regulatory). Interacts with alpha subunit ATP12A; this interaction is required for the formation of a functionally active pump and targeting at the plasma membrane (By similarity). Interacts (via N-terminus) with alpha subunit ATP4A (via the P-domain) (By similarity)

Gene: ATP4B  |  Organism: Homo sapiens  |  Synonyms: Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top