Recombinant Human Proteasome subunit beta type-2 (PSMB2), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 49.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Proteasome subunit beta type-2 (PSMB2)

Recombinant Human Proteasome subunit beta type-2 (PSMB2) — Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. Purity >90%.

SKU: BCRECP-001462 Category:
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Product Specifications

Product SkuBCRECP-001462
Product DescriptionRecombinant Human Proteasome subunit beta type-2 (PSMB2) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-201aa. [Accession | P49721].
Uniprot No.P49721
Gene NamesPSMB2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-201aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight49.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins.

Biological Context

Subcellular Location: Cytoplasm; Nucleus

Product Specifications

Recombinant Human Proteasome subunit beta type-2 (PSMB2) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-201aa, with N-terminal GST-tagged tag, molecular weight 49.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex)

Subcellular Location

Cytoplasm; Nucleus

Subunit

(Microbial infection) Interacts with HIV-1 protein Tat

Gene: PSMB2  |  Organism: Homo sapiens  |  Synonyms: Macropain subunit C7-I; Multicatalytic endopeptidase complex subunit C7-I; Proteasome component C7-I; Proteasome subunit beta-4
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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