Recombinant Human Pyridoxal kinase (PDXK), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 62.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Pyridoxal kinase (PDXK)

Recombinant Human Pyridoxal kinase (PDXK) — Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5′-phosphate (PLP), pyridoxine 5′-phosphate (PNP) and pyrido… Purity >90%.

SKU: BCRECP-001366 Categories: , ,
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Product Specifications

Product SkuBCRECP-001366
Product DescriptionRecombinant Human Pyridoxal kinase (PDXK) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-312aa. [Accession | O00764].
Uniprot No.O00764
Gene NamesPDXK
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-312aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight62.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

Biological Context

Subcellular Location: Cytoplasm, cytosol
Tissue Specificity: In adult testis and spermatozoa
Disease Association: Neuropathy, hereditary motor and sensory, 6C, with optic atrophy (HMSN6C) : An autosomal recessive neurologic disorder characterized by childhood onset of axonal, sensorimotor polyneuropathy affecting mainly the lower limbs, and adult-onset optic atrophy. Clinical features include progressive distal muscle weakness and atrophy, significant standing and walking difficulties, areflexia, neurogenic pain and progressive visual impairment. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1

Product Specifications

Recombinant Human Pyridoxal kinase (PDXK) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-312aa, with N-terminal GST-tagged tag, molecular weight 62.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503, PubMed:9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions

Subcellular Location

Cytoplasm, cytosol

Disease Association

Neuropathy, hereditary motor and sensory, 6C, with optic atrophy (HMSN6C) : An autosomal recessive neurologic disorder characterized by childhood onset of axonal, sensorimotor polyneuropathy affecting mainly the lower limbs, and adult-onset optic atrophy. Clinical features include progressive distal muscle weakness and atrophy, significant standing and walking difficulties, areflexia, neurogenic pain and progressive visual impairment. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

In adult testis and spermatozoa

Subunit

Homodimer

Pathway

Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1

Gene: PDXK  |  Organism: Homo sapiens  |  Synonyms: Pyridoxine kinase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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